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If working in guide manner and making use of bcl2fastq or BCL Change, or uploading the sample sheet to BaseSpace as a manual method operate and attaching a sample sheet, enter the i5 sequence during the forward path.
This permits massive numbers of libraries to generally be pooled and sequenced concurrently in the course of a single operate. In comparison with other indexing techniques, one of a kind dual indexing mitigates index hopping by filtering hopped reads.
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When assessing index sets for shade balance, the sequence noticed from the instrument needs to be assessed, regardless of the orientation useful for the sample sheet. To the i7 index, always asses shade balance utilizing the "i7 bases for sample sheet" column orientation.
Library prep, enrichment, and probe panel reagents must be requested separately. Appropriate library prep kits: